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The CodonAlign 2.0 software (Barry G. Hall, Rochester, NY, USA) was used to align the nucleotide sequences on the basis of alignments of the corresponding amino acid sequences performed with the ClustalW 1.8.3 software [ 60].
Primers were designed on the basis of alignments of all influenza A full-length segment sequences that included the conserved ends and regardless of the host of the virus isolate available at the time of primer design.
These rice best hit coordinates were used as the basis of alignments of each of the seven barley chromosomes with the twelve rice chromosomes.
We obtained intergenic spacer sequence data for 27 of the positive samples; 6 samples were B. burgdorferi sensu stricto, 7 were B. burgdorferi sensu lato (both on the basis of alignments of 816 bp), and 14 were B. miyamotoi (on the basis of alignments of 503 bp).
On the basis of alignments of sequences determined from conventional PCR products and sequences available from GenBank, a set of specific primers for the generation of a 160-bp amplicon of the groEL gene of O. tsutsugamushi was designed using PrimerSelect Version 6.1 software (DNAStar, USA); forward primer, 5′-TTGCAACRAATCGTGAAAAG-3′; and reverse primer, 5′-TCTCCGTCTACATCATCAGCA-3′.
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The spatial distribution of TAF5, TAF6 and TAF10 was found similar in SAGA and TFIID and was used as the basis of alignment of the two complexes [20], [21].
A phylogenetic tree (Figure 6C) was constructed on the basis of alignment of the partial AcNOS amino acid sequence and the corresponding homologous regions of several invertebrate and vertebrate NOS.
The maximum-likelihood phylogenetic tree of SbMYB44 was constructed on the basis of alignment of the complete deduced polypeptide sequences of different MYB TFs.
Primers were designed on the basis of alignment of complete 18S rRNA gene sequences of EU1, B. divergens, and B. odocoilei.
Nevertheless, in certain cases, such exons could be delineated on the basis of alignment of the genomic sequences with the EST cDNAs.
SNPs were identified on the basis of alignment of Illumina reads generated from each of the genotypes against a reference in this case, the CcTA and respective counter genotype, allowing not more than two mismatches.
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