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The amino acid residues of the NuoCD subunit starting from Met190 have been extracted on the basis of alignment to the sequences of the Nqo4/NuoD subunit and homologues from several organisms.
To this end, iterative filtering steps were employed to remove 80-86% of the reads on basis of alignment to rRNA/tRNA (bacterial, archaeal and eukaryotic origin), to phiX, and to the Illumina adaptor sequences (Additional file 3: Table S2).
The amino acid residues of the NuoCD subunit from Met190 to Arg596 is referred to as the NuoD segment (or simply 'NuoD' for brevity) in the present work on the basis of alignment to the sequences of the Nqo4/NuoD subunit from several organisms.
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Potential splicing signals to express VP1 were identified on the basis of alignments to other protoparvoviruses and classic RNA splicing motifs.
The ALLPATHS-LG scaffolds were anchored onto the pseudomolecules on the basis of alignments to both the optical map and the genotyping-by-sequencing (GBS) map.
This allowed to compare sweet orange gene models (see Methods) and two custom genome annotation tracks, representing the 61 GST encoding transcripts we assembled, and 1,611 ESTs putatively encoding GSTs on the basis of their alignment to genomic sequences.
We curated start sites on the basis of BER alignments to other, non- Blochmannia genomes.
On the basis of alignment, polymorphic loci against the reference sequence were identified according to the following criteria: Q ≥ 20, 3 ≤ Depth ≤ 100 and at least 5 bp away from each other.
Given millions of sequences, the goal is to identify all pairs of sequences that are highly similar (or "homologous") on the basis of alignment criteria.
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The spatial distribution of TAF5, TAF6 and TAF10 was found similar in SAGA and TFIID and was used as the basis of alignment of the two complexes [20], [21].
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