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Proteins from the query network are matched to the data repository on the basis of accession numbers such as UniProt (The UniProt Consortium, 2010) or Ensembl (Flicek et al., 2010).
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For non-referenced cultivars, the origins were estimated on the basis of the accession origin.
Primers were designed on the basis of the accession numbers from the microarray and confirmation of homology using BLAST (NCBI).
Sets of common probes between the different microarray platforms were established on the basis of sequence accession numbers and/or sequence comparison.
During phylogenetic analysis, sequences of the isolates were aligned on the basis of translated nucleotide sequences (accession NOs KR136279-R136303 and KR527208-KR527215), aneighbor-joiningining tree with p-distance was inferred with 1000 bootstrap replicates in MEGA version 5 for a 1485-nt fragment spanning the full gene of the envelope glycoprotein.
System-level function categories were assigned to the human genes in the subfamilies according to the following criterion: if, on the basis of microarray data (GEO accession ID: GDS596), a gene is specifically expressed in at least one of the tissues or organs represented in a system-level function category, then that system-level function category was assigned to the gene.
On the basis of the geographical distribution of accessions, the Yellow River basin (Huanghuai) is the most likely centre of diversity where allelic variation, especially as the complete deletion of mature miR1511 in miR1511-InDel-1a, was observed mostly in that region (Fig. 3).
The strain was identified on the basis of D1/D2 sequences analysis with accession number EU258671.
The CaSR primers and probe were designed, intron-spanning, using Primer Express 3.0 (Applied Biosystem) on the basis of equine CaSR mRNA (GenBank accession no.
Primers were designed to amplify all nine HPRT gene exons and flanking intronic sequences by polymerase chain reaction (PCR) (see Supplementary Table 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2014/290325), on the basis of the known genomic sequence (accession number NM 000194).
Hd1 was the first heading date QTL cloned on the basis of natural variation in rice accessions [ 10].
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