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We infer from the results presented here that a simple model [eq. (1)] developed on the basis of a quantification of forces destabilized during melting shows satisfactory performance for any length of the oligonucleotide sequence, salt concentration and base composition.
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These issues would have to be addressed if immunoEM studies are to form the basis of an accurate quantification of Ras clustering.
On the basis of the approximate quantification, precise quantification was performed using qPCR as recommended by the protocol following internal guidelines for submission of sequencing samples at the Genome Institute of Singapore.
The latter experimental condition was determined on the basis of the quantification of these two oxysterols in a few postmortem samples of brains with different levels of Alzheimer pathology within a pilot analysis that might suggest their increasing trend in the AD brain frontal cortex with disease progression.
On the basis of qPCR quantification with probes specific to the ends of the adapters (KAPA Biosystems), libraries were normalized to 2 nM, and then denatured using 0.1 N NaOH on the Perkin-Elmer MiniJanus.
Its tumor uptake was ∼8.2%ID/cm in the U87MG glioma and 3.5%ID/cm in the MDA-MB-231 breast tumor on the basis of SPECT quantification.
A second, quantitative system based on fluorogenic galactosidase activity confirmed the ZmHP2 stable interaction, and indicated, on the basis of the quantification of galactosidase activity, that the interaction of ZmHP2 with ZmTCRR-1 is more stable than with ZmTCRR-2.
On the basis of the quantification using the mass balance equation, the limit of detection of phosphorylation was 5% for SSANYR and 25% for ISASR when defined as 3 standard deviations from the mean measurements.
Next, these authors utilized SRM-MS assays to reliably quantify the 39 protein orthologs (selected on the basis of consistent quantification) in the sera of prostate cancer patients and controls, and used the resulting profiles to build predictive regression models for the diagnosis and grading of prostate cancer.
Standard deviation between groups was calculated on the basis of a previous experiment for CD11b stained area quantification, where σ = 19, thus yielding n = 6.34.
Comparative quantification was performed on the basis of a 2−ΔΔCt calculation method [ 18].
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