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The Evolution Theory speculates that two queried proteins may have a common evolutionary ancestor and serve as the basis for sequence alignments, which determine whether there exists sufficient similarity between two sequences.
Among these samples, 131 (10.6%; patient ages 1 132 months, median 12 months, mean 19 months) were positive for HBoV1, 5 (0.4%; patients 1 113 months of age, median 7.2 months, mean 29.2 months) for HBoV2, and 5 (0.4%; 1 108 months of age, median 12.0 months, mean 30.4 months) for HBoV3 on the basis of sequence alignment and phylogenetic analysis of PCR amplicons.
Geneious version 6.1.6 (Biomatters Limited) was used for sequence alignment and visualization.
We used the 5.05 version of MEGA5 software for sequence alignment (http://www.megasoftware.net/ 37.net/ 37
For sequence alignment, the CLUSTAW was used.
FASTA is used for sequence alignment in 3dRPC.
for sequence alignment.
For sequence alignment we used ClustalX [ 45].
To overcome this problem we used multiple structure alignments, when available, as a basis for multiple sequence alignments.
We used this structural alignment as a basis for the sequence alignments shown in Fig. 1f and Additional file 1: Figure S1, S4, as well as for superposition of multiple structures in Additional file 1: Figure S5.
On the basis of the multiple sequence alignment, we estimated the rate of evolution for each CP sequence in order to infer the selective constraints.
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