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Genetic and biochemical studies of the biosynthetic machinery have provided the basis for pathway engineering and combinatorial biosynthesis to create new or hybrid nucleoside antibiotics.
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We then selected ATCC #20362 strain for pathway engineering.
Moreover, since the design of the operon enabled more convenient DNA manipulation, pAM25 was used as a basis for all subsequent pathway engineering.
In particular, other biotechnological approaches, such as genome sequencing, functional genomics, genome engineering and omics will also provide a basis for pathway or genome reconstruction to improve its fitness and robustness for environmental stress [ 160, 161].
This result demonstrates that the effects of downstream pathways are important for MEP pathway engineering.
These microRNAs and the miR-297 669 miR-297 669 promising targets for apoptosis pathway engineering.
In conclusion, we first showed that carotenogenic enzymes can be targeted into the specific cellular location of recombinant hosts and this targeting strategy can serve as the basis for the subsequent development of sophisticated pathway engineering in microorganisms.
Systems biology, metabolic engineering and 'omics' technologies have paved the way for protein and pathway engineering.
Controllable regulation of gene expression is essential for either native pathway engineering or heterologous function introduction.
In addition, systematic engineering, including host and pathway engineering for terpenoid overproduction was reviewed.
Finally, systems biology, metabolic engineering, and "omics" technologies (genomics, functional genomics, and metagenomics) have opened the way for protein and biomolecular pathway engineering.
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