This high coefficient, observed despite the single cell bottleneck and the approximately seven generations of growth in liquid medium between the two measurements, is indicative of a heritable, genetic basis for colony size.
Interesting questions are presented by the interconnection of the biosyntheses of terpenoid hydrocarbons and very long chain fatty acids with the formation of the extracellular matrix materials in B. braunii that provide the building blocks for an extracellular carbon storage organ, but also the physical basis for colony organization in this organism.
In 1890 the BSAC invaded Mashonaland with a force of "Pioneers," and in 1893 it attacked the Ndebele kingdom, Matabeleland, creating the basis for the colony of Southern Rhodesia (now Zimbabwe).
Given the limitation of having one person collecting the data, pairs of colonies (replicates) were rotated on a daily basis; for example, colonies 1 and 2 were sampled on days 20, 23, 26, etc. while colonies 3 and 4 were sampled on days 21, 24, 27, etc. and colonies 5 and 6 were sampled on days 22, 25, 28, etc. Six rounds of collections took place over 3 weeks.
H. pylori was identified on the basis of colony morphology, gram staining and positive reactions for oxidase, catalase, and urease.
Bacterial colonies were selected on the basis of colony morphology and pigmentation.
After incubation at 37°C for 24 hours, Lac - and Lac + colonies were scored on the basis of colony color (Lac -: white, Lac+: pink).
After incubation, colonies were counted and identified on the basis of colony morphology, colony fluorescence, Gram stain and biochemical reactivity.
MAP was preliminarily diagnosed on the basis of colony morphology (small, smooth to slightly rough, opaque to whitish colonies).
After incubation, colonies of campylobacters were counted on the basis of colony morphology and typical cell motility (phase-contrast microscopy).
Isolates were identified on the basis of colony pigment, Gram staining, and catalase activity.
