In contrast to visual reading, measurement of quantitative fluorescence signals for ANCA IIF pattern interpretation provides for the first time the basis for objective assay performance assessment by determination of assay variability.
We established a stable T-cell line, which will provide the basis for an assay that facilitates monitoring of HIV-1 PR activity, as outlined in Fig. 1.
We exploited this property of the Gal4 system to express a reporter gene dependent on PR activity, the expression of the reporter gene being inversely proportional to the autocatalytic activity of PR, thus serving as the basis for our assay.
While we might have hoped that a simple assay could be used independent of the nature of the radiation exposure, it still could be feasible to employ a collection of signatures as the basis for an assay that assessed the nature and extent of a radiation exposure.
The basis for this assay is the use of a green fluorescent dye (CyQUANT GR dye), which exhibits strong fluorescence enhancement when bound to cellular nucleic acids.
We evaluated a new miRNA profiling platform that utilizes Illumina's existing robust DASL chemistry as the basis for the assay.
The basis for the assay is the distinct UV/Vis spectrum of quinone aniline adduct that is formed by the stoichiometric Michael addition of the amino component to the ortho-benzoquinone, formed by the oxidation of catechol by HRP.
Based on the allele frequencies estimated from our large sample of rice germplasm, researchers can select subsets of verified SNPs that segregate in specific subsets of genetic materials as the basis for targeted assays designed for QTL mapping, pedigree analysis, tests of hybrid or varietal seed purity, marker-assisted selection, etc.
The basis for these assays evolved from the IPA focusing on canonical lipid pathways that were significantly influenced by changes in PQQ status.
It is well known that antibodies can bind to specific antigens and this ability has been used as the basis for many assays [ 19- 21].
