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Consensus contigs developed across all 27 genotypes served as the basis for alignment to detect SNP.
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A minimum of 5 nt overlap per flanking junction sequence was required for alignment to be considered, selected on basis of maximizing overall alignment sensitivity (data not shown).
Other basic parameters like number of reads per contig as well as the average coverage were comparable, constituting a suitable basis for alignment analyses of contigs.
Recently, we proposed to use spaced words, defined by patterns of match and don't care positions, as a basis for alignment-free sequence comparison (Boden et al., 2013).
We curated start sites on the basis of BER alignments to other, non- Blochmannia genomes.
Using multiple reference sequences as a basis for the alignment of the candidate sequences significantly improves alignment quality.
To check sensitivity of additional taxon sampling from a number of the lophiiforms to the results reported in Yamanoue et al. [ 27], we used their pre-aligned sequences as a basis for further alignment with the 33 sequences.
To check sensitivity of additional taxon sampling of a number of the lophiiforms to the results reported in Yamanoue et al. [ 27], we used their pre-aligned sequences as a basis for further alignment with the newly determined sequences from 33 lophiiforms.
While this method is useful for functional alignment and decoding, it may also provide a basis for brain-to-brain communication using the converted pattern for designing brain stimulation.
The effects on the beam quality are illustrated providing the basis for the alignment procedure and jitter tolerances.
Some basis for community to coalesce around.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com