Four more diets were formulated with varying mixtures of these three ingredients, one included 33% of each ingredient on a dietary nitrogen basis (diet 33FSG) and the other three included a proportion 50 25 25 for each of the three ingredients (diets 50FSG, 50SGF and 50GFS).
Mixed rumen enzymes were prepared from rumen fluid collected from two rumen cannulated cows fed a mixed alfalfa hay (30%), barley silage (50%) and barley grain (20%; DM basis) diet.
In the digestion trial, 12 wethers (four per treatment) were fed 76% silage (DM basis) diets.
In a palatability trial, six mature wethers were fed 50% silage (DM basis) diets in a replicated 3 × 3 Latin square design with 28-d periods.
On a dry matter (DM) basis, diets contained 25% corn silage, 20% alfalfa silage, 10% cottonseed, 26.7 to 37% corn grain, and 4 to 13.5% protein supplement.
Dietary treatments were as follows: diet 1, barley straw (no supplement), diet 2, barley straw supplemented with 1% urea (dry matter basis), diets 3, 4, and 5 barley straw supplemented with 150, 300, and 450 g of alfalfa hay, respectively.
On a dry matter (DM) basis, diets contained 25.0% corn silage, 20.0% alfalfa silage, 10.0% cottonseed, 26.7 to 37.0% corn grain, and 4.8 to 13.5% protein supplement, plus minerals and vitamins.
The animals were distributed in a randomized block design and assigned to one of the isonitrogenous (18.4% CP, DM basis) diets which were formulated in forage:concentrate ratio of 40 60.
On a dry matter (DM) basis, diets contained 15% alfalfa silage, 25% corn silage, 34.3 to 36.9% corn grain, 19.4% soy products, 18.2% crude protein, 25.5% neutral detergent fiber, and 35.3% starch.
Following weaning on postnatal day 20, mice were divided into three treatments on the basis of diet supplementation (Normal rodent diet, 1% and 3% creatine supplemented diet) for 10 week.
Two repeated measurements of CH4 yield were conducted, the first measurement while sheep were fed fresh cut perennial ryegrass pasture (grass), the second with the same sheep fed a 400 600 concentrate:forage (wheat grain:lucerne hay; fresh basis) pelleted diet (pellet).
