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The temperature and pH (a measure of acidity or basicity) of the solution must be well controlled.
Indeed reduction of water within the electrospray capillary progressively increased the basicity of the solution, through formation of OH− ions, thus altering the charge state of the different proteins and improving the detection in negative ESI-MS.
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Due to the high basicity of the CHA solution (pH = 12.5), zinc ions react with the hydroxide ions and form different hydroxyl complexes such as [ZnOH]+, [Zn(OH 2](aq), [Zn(OH 3]- aq), and [Zn(OH 3]- aqq).
N, N′-diphenylbenzidine and its oxidation products, the corresponding semiquinoneimine and diphenoquinonediimine, dissolve as neutral molecules, monocations,or dications, respectively, depending on the acidity or basicity of the acetonitrile solution containing lithium perchlorate.
The distribution depends (Additional file 3) on the polarity and basicity of the solvent.
Each terminal O2− ion may in turn attach one or more H+ ions depending on its basicity and the pH of the solution.
The colour of the resulting lake depends on the degree of acidity or basicity the solution.
In order to study the effect of the solution basicity, the similar Co3O4 growth was also performed in an NH3 solution.
As the pH of final HAuCl4 solution was about 7, which was related to the basicity of the support and acidity of HAuCl4, pH of the solution would be not adjusted.
Upon removal of the chloride anion template, H NMR titration of the solution rotaxanes with a number of anions (chosen on the basis of their contrasting size, shape and basicity) showed selectivity for chloride anions over acetate anions and dihydrogen phosphate oxoanions, observations broadly supported by associated perturbations in osmium luminescence or redox character.
The acidity of the solution represented by the first equation is due to the presence of the hydronium ion (H3O+), and the basicity of the second comes from the hydroxide ion (OH−).
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