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The adopted method is basically described by Beamish [38] and Mayoral et al. [39].
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This has been basically described in vitro by Howell et al. [6] and in vivo by Iwata et al. [7], who reported human Aβ (hAβ) degradation through limited peptidolysis conducted by NEP.
Genome-scale models (GEMs) can be basically described as a network of metabolites that are connected by reactions occurring in the living organism.
PCMT was purified from transformed bacteria basically as described by MacLaren and Clarke 1995 [42], except that the original DEAE-cellulose chromatography final step, under non-equilibrium conditions, was replaced by Q-Sepharose HP chromatography, using a Hiload 26/10 column (Pharmacia, Uppsala, Sweden).
The expression of fusion protein was performed basically as described by Guan and Dixon (1991).
The infection of honey bee larvae was performed basically as described by [ 50].
The MS-PCR was performed basically as described by Herman et al (1996).
The RDA procedure was basically as described by Lisitsyn and coworkers [ 18, 21] using the BglII representation.
Plasma membranes were prepared from resting and germinating conidia basically as described by Burghoorn and co-workers [ 16].
Indirect enzyme-linked immunosorbent assay (ELISA) was performed to screen the sera for BTV-specific immunoglobulin G (IgG) antibodies basically as described by [ 6].
β-oxidation assays were performed using intact cells basically as described by van Roermund et al. (van Roermund et al., 2008) with slight modifications.
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