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ChIP assay was performed basically as described in the protocol of the ChIP assay kit from Active Motif (Cat# 53008&53009).
PCR was performed basically as described in the preceding section, except that one PCR tube of 20 μl containing 100 ng control DNA was employed.
Labeled RNA was used to hybridize the slides basically as described in [40].
The techniques followed were basically as described in Loarce et al. [ 25].
Agrobacterium-mediated transformation of P. digitatum Pd1 was conducted basically as described in [ 88] with minor modifications.
UVB Irradiation: UVB irradiation was carried out basically as described in previous studies.[ 18, 19] Briefly, SB2 cells were seeded at 60% confluency in 96 multiwell plates 24 h before irradiation.
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Endocytosis measurements were done basically as described [ 58].
The expression of fusion protein was performed basically as described by Guan and Dixon (1991).
The infection of honey bee larvae was performed basically as described by [ 50].
The MS-PCR was performed basically as described by Herman et al (1996).
TUNEL staining was performed basically as described (van Ham et al., 2010).
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