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To generate the reporter plasmid BMP6-Lux, pGL3 basic was used as the backbone.
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Empty pGL3-Basic was used as a negative control in all experiments and pGL3-Control was used as a positive control.
An antibody against myelin basic protein was used as a loading control.
In our study, radial basic function was used as the kernel function in SVM.
pGL3 basic vector was used as negative control and pGL3 control vector was used as positive control in the experiment.
Therefore, to contextualize the magnitude of OOP prices, the Tanzanian index amount of resources required for basic consumption was used as a reference for service affordability [ 11].
The pGL3-basic vector was used as a control in the luciferase assays.
The pGL3-basic vector was used as a baseline reference for all experimental wells with the results given as fold change of normalized luciferase signal over baseline.
The PGL3-basic-vector was used as a transfection control.
An empty pGL3-Basic vector was used as a control.
To measure the basal activity, an empty pGL3-Basic vector was used as a control vector.
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