After the fragments were cloned into the pGL3-basic vector, the vectors were sequenced to confirm that there were no wrong.
Figure 3A and B shows expression levels in Huh7 and smooth muscle cells, respectively, relative to expression of the pGL3 basic vector containing the LPL promoter only.
The subspace iteration method, which analyzes eigenvalue problems with the Jacobi method, is applied in the subspace formed by substitution of the total space basic vector with the subspace basic vector.
The TMV (tobacco mosaic virus) omega translation enhancer [ 49] was cut out of pGN35S-luc+ and inserted into the basic vector pluc using the restriction sites XhoI and NcoI to yield pluc-Ω (Fig. 1A).
Now, we choose the underlying five-dimensional lattice generated by the five mutually orthogonal vectors whose projections are the five basic vectors of the pentagon, as the geometric object that should be left invariant.
Wild-type and AP-1-binding-site-1-deleted promoters of MIR199A2 were amplified from HL-7702/L02 genomic DNA and cloned into the pGL3 basic vector (Promega) using the XhoI and HindIII site.
We first produced adequate experimental ASM vector data from the so-called Level 0 files containing the basic vector mode output of the ASM instruments (in engineering units).
The CaMV 35S long) promoter was inserted into the basic vectors using the restriction sites EcoRI and XhoI.
This assumption is reasonable, because the basic vectors of the Cartesian coordinate system satisfy it as well.
For, these elements are signed permutations of the n basic vectors generating the n-gon that transform into each other two of the other adjacent n-gons and put the third one in a new position.
Corresponding to the basic vector field V 1 in the optimal system, we can obtain only a constant solution.
