pGL3 basic vector was used as negative control and pGL3 control vector was used as positive control in the experiment.
The matrix of in an appropriate system of basic vectors is of the form (2.14).
The pGL3-basic vector was used as a control in the luciferase assays.
The pGL3-basic vector was used as a baseline reference for all experimental wells with the results given as fold change of normalized luciferase signal over baseline.
An empty pGL3-Basic vector was used as a control.
To measure the basal activity, an empty pGL3-Basic vector was used as a control vector.
The pLTR containing the HIV-1 5'LTR upstream of the luciferase gene in the context of the pGL3-basic vector was previously described [6].
Luciferase reporter assays using pGL2-Basic vector were performed in order to investigate the functional effect of mutations on DC-SIGNR promoter activity.
The empty pGL3-basic vector was used as control.
The pGL2-Basic vector was purchased from Promega (Madison, WI).
