Sentence examples for basic vector and from inspiring English sources
To improve speed, the current version of PROCOV makes use of the high quality routines in Basic Linear Algebra Subprograms (BLAS; ) implemented in Automatically Tuned Linear Algebra Software (ATLAS; ) to perform basic vector and matrix operations.
The cloning of promoter constructs in luciferase reporter vector, the pGL3 basic vector and the ASPM, CDK5RAP2, MCPH1 and WDR62 promoter constructs containing mutations in estrogen receptor binding sites were conducted using QuickChange II XL site-directed mutagenesis kit (Stratagene. La Jolla, CA, USA) following the recommended protocols.
Furthermore, they showed that the minimal sections are the ones orthogonal to vectors with only one non-zero coordinate, and the maximal sections are orthogonal to vectors of the form e j + σ e k, where j ≠ k, e j and e k are standard basic vectors, and σ ∈ C, | σ | = 1.
The PCR product was cloned between the XhoI and HindIII sites of the pGL3-basic vector and transfected into F11 neuronal cells using Lipofectamine 2000 as described previously [12] with control shRNA or shRNA specific to LMO4.
To rule out the possibilitythat Wnt4 non-specifically induced the pGL3-basic vector, we performed a control experiment in which we co-transfected cells with the pGL3-basic vector and either the HA-empty vector or the HA-tagged wnt4 vector.
pGL3-basic vector and pRL-SV40 luciferase reporter were purchased from Promega (Madison, WI, USA).
The pGL3-Basic vector and pGL-promoter vector were used as negative and positive controls, respectively.
The pGL3-Basic vector and pGL3-NF-κB-Luc were the same as described previously [ 29, 30].
We constructed the mouse MIP-1β promoter (-1,989 to +68) into pGL3-basic vector and determined whether AR can activate the MIP-1β promoter.
After 24 h of incubation, pGL3-basic (vector) and u-PA promoter plasmid were co-transfected with a β-galactosidase expression vector (pCH110) into cells using Turbofect (Fermentas, Carlsbad, CA).
