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This chapter first introduces vectors and then presents the basic vector algebra.
The basic vector pmirGLO served as an empty vector.
The basic vector pSIN18.cPPT.H1p.hEF1αp.RFP.WPRE served as an empty vector.
Briefly, to isolate SPANX-A1 and SPANX-A2 containing loci, the vector TAR-A was constructed from basic vector pVC604.
The CYP24 was cloned to the kpn/BgI II sites of the promoterless pGL3 basic vector (Promega, Madison, WI).
Data are expressed relative to the activity of the promoter-less pGL3 basic vector.
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The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity.
The pGL3-basic vector was used as a control in the luciferase assays.
PCR products were cloned into pGL3-basic vector and sequenced.
The pGL3-basic vector served as the negative control.
An empty pGL3-Basic vector was used as a control.
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