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This protocol describes the basic steps involved in conventional plasmid-based cloning.
Those familiar with Microsoft Word's macro tool will recognize the basic steps involved in creating one: press Record, push the buttons you wish to include, press Stop.
In this talk I explain the basic steps involved in tranforming a controller, defined as a mathematical object, into a real-time program that realizes it.
In this talk I explain the basic steps involved in transforming a controller, defined as a mathematical object, into a real-time program that realizes it.
Thanks to efforts such as these, the basic steps involved in regeneration have been established in the organs of several types of animal; these include the zebrafish heart, salamander limb and mammalian liver.
It presents the basic steps involved in building synthesis in a new language, which include defining a phone set, defining a lexicon, designing a database to record, recording the database, building the synthesizer, text normalization, creation of prosodic models, evaluation and tuning, and addressing language-specific issues.
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In OR, there are three basic steps involved to select and prioritize the forwarders list.
It presents the basic intermediate steps involved in the derivation of a solution methodology, from a scientific motivation to the numerical results, which can be applied for both steady and transient problems, even when the boundaries have an arbitrary shape.
There are two basic iterative steps involved in the process: the creation of diversity at the gene level and the screening or selection for improved variants [reviewed in [ 1- 3]].
However, in order to incorporate necrosis not only phenomenologically, as in Pérez-García et al. (2011), it is necessary to take into account the basic intervening steps involved in palisade formation discussed in Brat et al. (2004) and Brat and Van Meir (2004).
The basic steps involve creating a 'wound' in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the wound and comparing the images to quantify the migration rate of the cells.
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