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When viewed with basic staining techniques, some types of lymphatic cancer cells have one distinctive morphology, are highly metastatic, and thus have the ability to spread throughout major anatomical features, while other types of cancer cells have a different type of morphology and are only able to spread through minor substructures of the lymphatic system.
Earlier classification systems were based on tumor size (microadenomas <10 mm vs. macroadenomas >10 mm) and basic staining characteristics (acidophilic, basophilic, chromophobic).
Gels were electrophoresed for 1 h at 150 V and LPS was visualized with the SilverQuest Silver Staining Kit (Invitrogen) using the basic staining protocol.
The basic staining procedure used an avidin-biotin-alkaline phosphatase method, modified for antigen retrieval from paraffin-embedded tissue using the procedure of Shi et al [ 36].
After transferring 5 μm sections onto MMI membrane slides, these were fixed in 70% isopropyl alcohol and then stained with the MMI basic staining kit.
The tissue specimens were processed routinely for light microscopy (fixation, dehydrating, embedding, and sectioning) and staining with hematoxylin and eosin (HE, basic staining) and van Gieson's stain (VG, nonspecific collagen staining).
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However, we do know that hematoxylin is a basic stain that colors nuclei and ribosomes blue/purple, and eosin is an acidic stain that colors protein-rich cytoplasm and extracellular matrix pink/red.
This is a common, basic, stain removal product available at many stores.
Thin layer chromatography (TLC) was performed using 60 mesh silica gel plates and visualization was performed using short wavelength UV light (254 nm) and/or basic KMnO4 staining.
Immediately after sacrifice and dissection the right femora were prepared for basic fuchsin staining and plastic embedding following previously established protocols.
Methylene blue-basic fuchsin staining.
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