Exact(12)
The N/N-LOX-1 mutant has either interrupted electrostatic potential and asymmetric fluctuations of the basic spine arginines.
Secondly, it was observed an asymmetric flexibility of the basic spine residues, in particular in the N/N-LOX-1 mutant.
An intermediate fluctuation difference is observed for the basic spine arginines of the K/N-LOX-1 monomers (the absolute value of their difference ranging from 0.02 to 0.04 nm) (Figure 7, panels C and D).
The G-to-C transition of LOX-1 gene results in the K-to-N change at 167, far from the basic spine arginines of the CTLD domain that represents the ligand binding domain (Figure 6D).
The fluctuations of the residues (i.e. the standard deviation of residue position during the simulation) have been also monitored from the MD simulations focusing the attention on the basic spine residues.
E170, as a consequence of mutation, remains as a free negative charge fluctuating on the LOX-1 surface, influencing the electrostatic potential generated by the arginines of the basic spine.
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The bad news: well, all the other things that were broken – undermanned defence, lack of basic spleen and spine and muscle (and brain) when it comes to keeping the opposition at bay – appear to be just about clinging on behind.
These spines are of two basic types, stiff central spines located in the middle of the areole or radial spines that grow out laterally from the edges of the areole; the former are probably protective or when brightly coloured attract pollinators, while the latter are often white and reflect sunlight, providing shade and protecting the plant body from solar radiation.
In the spine the basic functional unit comprises vertebrae, intervertebral disc and ligament tissues.
Complete another basic Pilates move, the spine twist.
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