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The rationale behind our basic protocol can be readily used to adapt other retroviral plasmids.
Several improvements of the basic protocol can be adopted in order to minimize possible sources of false-negative results, such as the inclusion of a reference probe to allow quantification of the non-expanded alleles.
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Real-time RT-PCR technology provides high sensitivity and accurate expression profiles [ 14, 15] and in that approach, two basic protocols can be followed: absolute quantification and relative quantification.
While the scoring functions discussed in the previous section are designed to detect family-defining positions (and catalytic positions in particular), this basic tree traversal protocol can be adapted to detect SDPs as well.
The basic iterative, synchronous consensus protocol can be defined as follows over iterations k: begin{aligned} {tilde{h}}_i^{ k+1)} = {tilde{h}}_i^{ k)} + xi sum limits _{jin N_i} left( {tilde{h}}_j^{ k)} - {tilde{h}}_i^{ k)}right) end{aligned} (15 where (xi) is the step size.
(That being that date when the Madrid Protocol can be reviewed by the treaty nations).
The text of the Kyoto Protocol can be found at //unfccc.int/resource/docs/convkp/kpeng.html.html
The protocol can be tuned by setting the protocol parameters.
This basic protocol can easily be adapted if required.
Several scheduling protocols can be used to guarantee basic real-time behavior.
More specifically, and taking into account that NeMHIP has been developed based on the HIP protocol, its basic goal can be expressed as follows: the protocol must not introduce security vulnerabilities to the network mobility management compared to the HIP protocol.
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