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In order to immunostaining eosinophils, we used antibody to major basic protein as a marker.
The intermediate filament proteins nestin, vimentin, and glial fibrillary acidic protein was used as markers for astroglial maturation and astrogliosis, and myelin basic protein as a marker for oligodendrocytes and myelin formation.
We next measured the phosphatase activity in avicin D-treated U266 cells using P32 labeled myelin basic protein as a substrate.
Because PA83 is resistant to the viral proteinase, the activity of the latter was determined using myelin basic protein as a substrate (Fig. 9) [33].
Using P32 labeled myelin basic protein as a substrate, the phosphatase activity of the immunoprecipitated PP1 was measured with the aid of the protein serine/threonine phosphatase (PSP) assay system.
DYRK 1B activity in immune complexes was assayed as reported, using myelin basic protein as a substrate.
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A study in rodents has demonstrated that aerobic exercise attenuates age-dependent alterations in a protein marker of myelin (myelin basic protein) as well as in astrocytes, which promote myelination via oligiodendrogial cells.
Importantly, hASCs expressed myelination master-gene Krox20 (Egr2) together with transcripts encoding components of peripheral myelin, including proteolipid protein 1, peripheral myelin protein 22, periaxin, 3 transcript variances of myelin basic protein as well as myelin proteins zero-like 1 and 2 (for details see Table S1).
Despite the separation power of this approach, 2DE MS-based proteomics presents some limitations such as a difficulty in detecting low-abundance, acidic or basic proteins as well as proteins with extremes of high or low molecular weight [ 2DE MS-based
We also quantified the expression of MAG (myelin associated glycoprotein) and MBP (myelin basic protein) as markers of mature oligodendrocytes and myelination parameters.
Immunocomplex kinase assays were performed by incubating the immune complexes in 40 µl of kinase buffer containing 2.5 mCi of [γ32P]ATP (Amersham Pharmacia Biotech Inc ., 25 mM ATP, and 1 mg of myelin basic protein as substrate.
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