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RNA was prepared from cell cultures using the same basic procedure used for intact tissues except that at the time of harvest, the cell layers were directly scraped up into Trizol® (Invitrogen Carlsbad, CA) reagent and either directly extracted or frozen at −80°C The basic technical approaches that were used in these studies are presented in Wang et al., 2006 [18].
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The apparatus and basic procedures used for temperature-programmed hydropyrolysis have been described in detail elsewhere.[3] Briefly, samples were mixed with acid-washed sand (1 5 w/w) and heated in a stainless steel reactor tube from 150 to 520°C at 8°C min-1 using a hydrogen pressure of 15.O MPa.
The measurement of the height of individuals, especially young men, became the basic procedure used to classify them as appropriate or not for military recruitment.
Watermark extraction follows the basic procedure used in embedding.
The procedure used for computing is detailed in the Supplementary Information.
Clearly, this part of pipeline follows the basic strategy and procedures used for phylogenetic method or tree-based ortholog identification [ 4, 10], with our own implementations, modifications and improvements.
The basic staining procedure used an avidin-biotin-alkaline phosphatase method, modified for antigen retrieval from paraffin-embedded tissue using the procedure of Shi et al [ 36].
The same basic procedure was used by all of the above-mentioned psychologists.
Here are the basic steps for the permutation procedure used in the lth iteration of the search algorithm.
Basic topology used for simulation.
This basic procedure was first used in its present form on the Detroit River Railroad Tunnel between Detroit and Windsor, Ontario (1906 10).
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