Sentence examples for basic medium supplemented with from inspiring English sources

The T98G, U-87MG, and A549 cell lines were purchased from the American Type Culture Collection (ATCC), the cells were cultured in DMEM basic medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified incubator at 37 °C and an atmosphere of 5% CO2.

The cells were then washed 3 times with saline, and aliquots of the washed cells were inoculated into 10 mL of basic medium supplemented with 4 g/L of urea and 10 to 50 g/L of carbon sources in 100-mL Erlenmeyer flasks which were then incubated at 30°C on a rotary shaker at 230 rpm.

At passage 5 10, the cells were transferred to hESC basic medium supplemented with 10 ng/ml bFGF.

The conventional and most frequently used hepatogenic medium is basic medium supplemented with growth factors including epidermal growth factor, bFGF, HGF, nicotinamide, oncostatin M, dexamethasone and ITS premix.

hMSC basic medium was composed of hMSC proliferative medium without bFGF; hMSC osteogenic medium was composed of hMSC basic medium supplemented with 10-8 M dexamethasone.

S. solfataricus P2 (DSM1617), PBL2025 [ 33] and Ss-lrpB::lacS[ 9] strains were grown aerobically at 80°C in Brock basic medium supplemented with 0.1% tryptone as a carbon and energy source [ 34].

COCs surrounded by compact multilayered cumulus were cultured in the above mentioned basic culture medium supplemented with 10% inactivated estrous cow serum (prepared in our laboratory) and 5 IU mL gonadotropins PG600 (Intervet, International B.V. Boxmeer, Holland) in 0.5 ml volume per one well, in 4-well culture dishes (Nunclon, Roskilde, Denmark) for 44 hours at 38.5°C, 5% CO2 and 7.5% O2.

To test adipogenic potential, TSCs were cultured in adipogenic induction medium (Millipore, Cat. # SCR026) consisting of basic growth medium supplemented with 1 μM dexamethasone, 10 μg/ml insulin, 100 μM indomethacin, and 0.5 mM isobutylmethylxanthine (IBMX).

Finally, the osteogenic potential of MMSCs and BMSCs was tested by culturing them in osteogenic induction medium (Millipore, Billerica, MA) consisting of basic growth medium supplemented with dexamethasone (0.1 μM), ascorbic 2-phosphate (0.2 mM), and glycerol 2-phosphate (10 mM).

As a test of chondrogenic potential, two kinds of MSCs were cultured in basic growth medium supplemented with prolin (40 μg/ml), dexamethasone (39 ng/ml), TGF-β3 (10 ng/ml), ascorbate 2-phosphate (50 μg/ml), sodium pyruvate (100 μg/ml), and insulin transferrin-selenious acid mix (50 mg/ml) (BD Bioscience, Bedford, MA).

The next day, the medium was replaced with regular human ES cell culture medium supplemented with basic FGF.