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Translational science in medicine describes the transfer of basic in vitro and in vivo research into human applications.
In summary, this research demonstrated that: (i) SIS gel can be used to create contractile engineered cardiac tissue; (ii) SIS gel produced engineered cardiac tissues with a more physiological contraction rate and higher phenotypic protein expression based on the basic in vitro examinations performed in this study.
Once basic in vitro testing identifies an optimal combination of a specific material and augmentation methods, transitioning these biomaterials to a clinical setting requires preclinical studies utilizing animal models that best recreate the clinical scenario in a reproducible and cost-efficient manner.
This study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods.
The goal of the present work is to establish a basic in vitro 3D spheroid model of human mesothelioma and to investigate how the tumor microenvironment affects the penetration and killing activity of the immunotoxin SS1P targeting mesothelioma.
Primary CML cells were collected after informed consent to the use for basic in vitro research, according to the Helsinki declaration and following approval of the Ethics Committee, at the Division of Hematology of Università di Firenze.
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The main priority for systems medicine is the development of computational models that integrate data and knowledge from the clinics and basic science (in vitro and animal model experiments) and are applicable to individual patients.
New drugs go through three basic testing phases: in vitro (test-tube) and in silico (computer) modelling; animal testing; and, finally, human trials.
The 3D porous scaffold with a concentration gradient of growth factors may become a useful tool for basic studies, including in vitro investigations of 3D chemotaxis/haptotaxis for the control of specific biological process.
Finally, although the long-term goal must be the engineering of blood vessel substitutes for clinical application, a more realistic short-term goal for tissue engineering is to develop more physiologic models for use in basic vascular biology in vitro studies.
Of note, integrin α-4 was not permanently expressed under basic culture conditions in vitro, but intermittently undetectable in the majority of positive preparations including the cell line.
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