However, most of these studies used insensitive methods of RNA detection (northern blot) [ 9, 11], or the methods were only semiquantitative [ 13, 14] or failed to relate specific gene expression to the basic expression level of housekeeping genes [ 9, 10].
Although the basic expression level of CXCL1 was generally higher in both disease entities in comparison to CXCL5, we detected that CXCL5 overexpression was significantly more pronounced compared to CXCL1.
On the other hand, we also observed a significantly higher CXCL1 expression level in the CRA tissues, where 4000 pg/ml CXCL1 compared to 200 pg/ml CXCL5, indicating that the basic expression level of CXCL1 is generally higher in both disease entities in comparison to CXCL5 (Fig. 5B).
This study investigated the basic expression levels and the ex vivo inducibility of hBD2 mRNA in peripheral whole blood cells from patients with severe sepsis in comparison to non-septic critically ill patients and healthy individuals.
The expression levels of basic fibroblast growth factor, vascular endothelial growth factor, interleukin-8 (IL-8), matrix metalloproteinases (MMP) 2 and 9 and E-cadherin were examined at the periphery of the tumour by a colorimetric in situ mRNA.
To obtain direct evidence that the Wnt/β-catenin signaling pathway can trigger extracellular matrix degradation and hypertrophic chondrocyte differentiation in osteoarthritis, we activated the pathway in vitro by using LiCl in normal and osteoarthritic chondrocytes and evaluated the expression levels of basic catabolic (MMP-7, 9, 13, 14, ADAMTS-5, and 4) and hypertrophic markers (collagen X).
After validating suppression of the Pmp22 mutant allele at the mRNA level in the Schwann cells of Tr-J mice, we observed increased expression levels of myelinating proteins such as myelin basic protein and myelin protein zero.
The expression levels of the FL construct were significantly increased compared with the pGL3-basic construct.
This multiplexed technology can simultaneously determine expression levels of multiple targets in less than 2 h using sample inputs compatible with many basic science and clinical detection applications.
