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The Tat peptide, penetrain and pVEC are short peptides (∼20-mers) derived from the basic domain of the HIV-1 trans-activator of transcription (Tat) protein, the third helix of the Antennapedia homeodomain and cadherin, respectively.
It was demonstrated that an arginine to isoleucine mutation in the basic domain of the yeast bZIP factor GCN4 completely inhibited its affinity for the AP1 site [ 57].
This system consisted of a dexamethasone-inducible cell line that expressed a reporter protein (GFP) fused to the carboxy-terminal basic domain of the rice calmodulin (CaM61), which naturally bears a CaaL geranylgeranylation motif (GFP-BD-CVIL).
The B3 domain is a plant-specific basic DNA-binding domain originally identified as the third and C-terminus proximal basic domain of the transcription factor ABSICIC ACID INSENSITIVE3 (ABI3) (reviewed in [ 21]).
Previously, it has been reported that the basic domain of the HIV-1 transactivator of transcription (Tat) protein, which is composed of 11 amino acid residues, possesses the ability to traverse biological membranes efficiently in a process termed 'protein transduction' [ 9, 10].
In a preceding study we have recently established an in vivo visualization system for the geranylgeranylation of proteins in a stably transformed tobacco BY-2 cell line, which involves expressing a dexamethasone-inducible GFP fused to the prenylable, carboxy-terminal basic domain of the rice calmodulin CaM61, which naturally bears a CaaL geranylgeranylation motif (GFP-BD-CVIL).
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For example, functional divergence between Ato and Neurog proteins in Drosophila is encoded by three non-conserved residues in the basic domain of these bHLH which are responsible for the differential interaction with Senseless or MyT1 [ 59].
More specifically, disease-targeted and generic HRQoL domains were found to be well correlated with HU scores, especially for domains related to pain, physical function, and emotional and mental health, which are basic domains of the EQ-5D and HUI utility scores.
In contrast to the N-terminal basic domain of TRF2, the N-terminus of TRF1 is rich in acidic residues and appears to inhibit the ability of TRF1 to induce supercoiling, as deletion of the acidic domain (TRF1ΔA) enhances the ability of TRF1 to promote supercoiling.
Likewise, replacement of the basic domain of TRF2 with the acidic domain of TRF1 (TRF2hAΔB) weakens the ability of TRF2 to promote supercoiling.
As a consequence, the basic domain of study in the social sciences consists of social practices ordered across space and time.
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