Sentence examples for basic culture medium for from inspiring English sources

The basic culture medium for oocyte maturation was Tissue Culture Medium199 (Sigma, St . Louis MO, USA), supplemented with 10% patient's serum, 0.3 mM pyruvate (Sigma; St . Louis MO), 0.075 IU/ml FSH and 0.5 IU/ml HCG (Serono, Rome, Italy).

The cells were cultivated in a basic culture medium of Dulbecco's modified Eagle medium (DMEM /F12 supplemented with 10% fetal bovine serum (FBS) and 40 ng/ml basic fibroblast growth factor (bFGF).

The other half of the cells was washed twice with DPBS and culturing was continued for 8 days with basic culture medium.

A basic culture medium is described in Table  21.

Renew the medium every day thereafter with the same volume of basic culture medium.

After gel polymerization, add gently an appropriate amount of pre-warmed basic culture medium.

These evaluations suggested that the basic culture medium should be supplemented with 5%% plasma.

The basic culture medium was replaced every 3 or 4 days.

Fresh basic culture medium was supplied to the cell pellet, which was then resuspended.

For organ culture investigations, 1 woman and 14 men, aged 22 to 48, donated skin; samples were collected into sterile universal tubes (25 or 50 ml) containing basic culture medium: William's E medium supplemented with 10 μg/ml insulin, 10 ng/ml hydrocortisone, 2 mM l-glutamine (Life Technologies, Paisley, UK), and 10 U/ml penicillin.

This was necessary since the previously described liquid overlay method used RPMI culture medium for basic cell line culture, this was replaced by DMEM/F12 to better accommodate primary tumor cells.