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COCs surrounded by compact multilayered cumulus were cultured in the above mentioned basic culture medium supplemented with 10% inactivated estrous cow serum (prepared in our laboratory) and 5 IU mL gonadotropins PG600 (Intervet, International B.V. Boxmeer, Holland) in 0.5 ml volume per one well, in 4-well culture dishes (Nunclon, Roskilde, Denmark) for 44 hours at 38.5°C, 5% CO2 and 7.5% O2.
BJ cells were cultured in 75 cm culture flasks containing basic culture medium: 86% of E-MEM medium supplemented with 10% fetal bovine serum (Sigma-Aldrich, St . Louis MO, USA), 1% Sodium Pyruvate, 1% non-essential amino acids, 1% GlutaMAX and 1% antibiotics (GIBCO, Carlsbad, CA, USA) until the time of treatment.
The basic culture of chastity is in no way being publicly rethought.
Safter Cinar, the head of an association of Berlin's 130,000 Turks, says such unions remain "the basic culture, the usual pattern".
Many things have changed since those days, but the basic culture of an independent police in Britain has not – or not yet.
The basic culture of these peoples survived the Roman occupation and was later strengthened and broadened by Celtic immigrations from other parts of Britain.
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To find the place for you, you need to understand the basic cultures and topics prevalent on these platforms". When these advantages of data are combined, it presents Australian startups with a unique opportunity.
The cells were cultured either in basic cell culture medium or in osteogenic medium (OM) supplemented with 50 mg/ml AA (Sigma-Aldrich, St .Louis, Missouri, USA) and 7.5 mM β-GP (Sigma).
But the French bishops' language of "basic religious culture" captures a reality that the International Theological Commission's treatise misses.
Training in the engineering and design industry needs to cover seven basic areas workplace culture, technical (basic and advanced skill) knowledge, project-specific technical training, and company-specific safety training.
In it, they found much stronger language for such institutionalized evils, referring, in connection with anti-Semitism, to "an underlying basic religious culture which shaped and deformed people's attitudes".
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