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Firstly, the basic cluster synchronization concept in synchronous Boolean network is provided and necessary and sufficient conditions are derived.
In addition, the corresponding information is added into theTBL F. Step 8: Go to Step 2. The cluster, after Tabu Search, has the basic cluster characteristics.
The MEKK3-PB1 domain (type II group) contains a positively charged basic cluster in the front end, whereas the MEK5-PB1 domain (type I group) contains a negatively charged acidic OPCA motif in the back end.
An even more pronounced phenomenon was observed when IFNγ basic cluster D2 was modified, with a single mutation at position 140 almost completely abolishing LcrV recognition.
The KKR N-terminal basic cluster of LukS-PV signal peptide constitutes a good candidate for heparan sulfates binding site [25].
Analysis of the flanking sequences in EBNA-1 revealed a downstream "PRR" motif that is also found in the Region II basic cluster of KSHV LANA, indicating that the EBNA-1 NLS also matches the bipartite consensus (Fig. 8C).
Notably, the basic clusters of IFNγ (and specifically the GRRA138 141 motif, which overlaps the second basic cluster) play a role in LcrV recognition in cell culture conditions [28].
Individual proteins maybe identified that have a preference for either the acidic or basic cluster.
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A basic cluster-association matrix BM is generated at first based on the crisp associations between samples and clusters using HBGF, in which there are n samples and m× k clusters.
In previous studies, the structure of the NLS Karyopherin α complexes have been described for NLSs of the classic single-basic-cluster type [e.g. SV40 T antigen (36); androgen receptor (37)] and bipartite-cluster type NLS [e.g. Nucleoplasmin (38, 39)].
A dot plot alignment of the terminase gene products (TerL) confirmed our basic cluster/subcluster assignment with 100% of phages grouping by their pre-assigned clusters or subclusters, and 11 of 14 singletons remaining singletons.
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