Primarily, basic cellular and metabolic processes were enriched, such as amino acid or nucleic acid metabolism.
Our analysis of the gene ontology functions of this group showed an enrichment of genes related to maintaining basic cellular and metabolic processes.
Others affect basic cellular and metabolic processes, or are mutations in computationally predicted genes for which aggressive behavior is the first biological annotation.
Analysis of GO categories reveals that while some of the genes in this core set are involved in spermatogenesis or oogenesis, the overrepresented GO terms indicate a role in basic cellular and metabolic functions.
While many of the genes affect the development and function of the nervous system, and are thus plausibly relevant to the execution of complex behaviors, others affect basic cellular and metabolic processes, or computationally predicted genes for which aggressive behavior is the first biological annotation.
The budding yeast, Saccharomyces cerevisiae, has been an excellent eukaryotic model system for understanding basic cellular processes and metabolic pathways [ 1]. S. cerevisiae was the first eukaryotic genome for which the genome sequence was reported [ 2].
However, GO analysis of genes over-expressed in GBM compared to normal brain tissues revealed enrichment of general GO terms in basic cellular metabolic and biosynthesis processes such GO 0031328 positive regulation of cellular biosynthetic process, GO 0045941 positive regulation of transcription, GO 0010467 gene expression.
These genes were involved in diverse biological processes and those involved in basic cellular, biosynthetic, catabolic, and metabolic activities, as well as responses to stress and signal transduction, were highly enriched.
Many of these processes were over-represented in both the in vitro and field graft transmitted genes, covering various basic cellular, biosynthetic, catabolic, and metabolic activities.
Gene Ontology (GO) term analysis of the 3333 graft transmitting genes, using the Plant MetGenMAP analysis tool [ 34], indicated that diverse biological processes were over-represented including those related to many basic cellular, biosynthetic, catabolic, and metabolic activities, as well as responses to stress and signal transduction (Additional file 2: Dataset S3).
In addition, WSSV is predicted to affect several basic cellular metabolic processes, including oxidative phosphorylation, protein synthesis, glycolysis, and calcium ion balance.
