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Some basic cell sorting of Cre-expressing and/or lineage-marked cells following by staining of cytospins and gene expression analysis would rapidly enable the unipotent stem cells/lineage-committed progenitors/pregnancy-responsive progenitors to be correlated with one of the epithelial subpopulations that flow cytometry has enabled us to isolated and characterise.
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Left: Cell sorting of 800 cells.
Thus, further studies using the experimental model described here should help link the basic cell biological processes of endocytic traffic, sorting and recycling of such receptors to the pathogenesis of endothelial injury which is an integral part of a number diseases of substantial human health importance.
This suggests the possibility of using optical deformability as an inherent cell marker for basic cell biological investigation, diagnosis of disease, and sorting of stem cells from heterogeneous populations, obviating the need for external markers or special preparation.
Recommended: basic physics, basic cell biology, and consent of instructor.
For cell sorting, the cells of particular sizes were sorted according to their forward-scattered light intensity.
Finally, we demonstrated on-chip cell sorting as an application of the microvalves by sorting cells using the proposed gel-valves.
Two rounds of cell sorting were performed.
Fluorescence-activated cell sorting confirmed the presence of CD133+ cells.
Currently, the most widely used methods of cell sorting are fluorescence-activated cell sorting (FACS) and magnetically-activated cell sorting (MACS) [ 36- 38].
The GFP-positive p185wt cells (p185wt GFP cells) were sorted by fluorescence-activated cell sorting to a purity of >95%.
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