By introduction of pH-responsive fluorophore 5 6 -Carboxyfluorescein (CF), above pH variation could be easily detected through fluorescence (FL) spectrometter, which could be utilized to determine the variety of amino acid enantiomers (including basic and acidic amino acids).
The amino acids were grouped as basic (Lys, His, and Arg), acidic (Asp, Glu, and Asn), charged (basic and acidic amino acids), hydrophilic (charged amino acids, Thr, and Ser), hydrophobic (Val, Leu, Ile, Phe, Tyr, Trp, and Met), and apolar (hydrophobic amino acids except Tyr) [ 41].
Basic and acidic amino acids, as well as stretches of different amino acids are highlighted in the N-terminal region.
The pKas of basic and acidic amino acid sidechains can be significantly altered in the enzyme environment.
But, CaZF has two such combinations of basic and acidic amino acid stretches.
A close analysis of the amino acid sequence reveals that this domain contains apart from alternate stretches of basic and acidic amino acids, a potential site for protein kinase C phosphorylation (SKK) and a potential site for cAMP/cGMP-dependent protein kinase phosphorylation (KKKS).
Amino acids are color coded: nonpolar, uncharged polar, basic, and acidic.
The EMBOSS application Pepinfo [ 49] was used to calculate amino acid class (Aliphatic, Aromatic, Non-polar, Polar, Charged, Basic and Acidic) percentage compositions and hydrophobicity values based on the method of Kyte and Doolittle.
However, these materials are chemically unstable, which can gradually dissolve in strong basic and acidic solutions.
Occurrence of a large column of basic and acidic tuff and.
Experimental diets' basic and acidic degree of hydrolysis was evaluated in vitro.
