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The default settings for Primer3 program: optimum temperature of 63oC and an optimum primer size of 24 bases were selected for designing the primers.
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To focus on more differentially expressed genes, 1125 genes with variation in expression among tissues equal or larger than 3.24 fold (1.8 under log2 base) were selected for analysis.
Based on this compilation the BACs were selected for validation studies.
All indel sequences surrounded by at least 10 bp of perfectly aligned flank and with no more than 50% undetermined bases (Ns) were selected for further analysis (Table 1).
On the other hand, polydimethylsiloxane (PDMS) based membranes were selected for their stability in these polyglycols and for their marked affinity for DCM.
Quantitative structure activity relationship based features were selected for predicting inhibition activity of a compound against HIV proteins namely protease (PR), reverse transcriptase (RT) and integrase (IN).
Using the GS FLX pyrosequencing software, high-quality sequences (> 99.5% accuracy on single base reads) were selected for further processing and assembly.
A number of transcripts shown to undergo changes in the 1 week microarray experiment and/or known to be involved in acid-base regulation were selected for analysis by qRT-PCR, according to their relevant function and statistical significance.
This is noteworthy, since all previous pEPI-1 based vectors were selected for the NPT gene, present in the second dispensable SV40-O/P driven transcription unit [ 1, 3, 4].
As a result, the 10000 compounds with top scores during the pharmacophore based virtual screening were selected for the further molecular docking.
The top 10 peptides, based on intensity, were selected for fragmentation.
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