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Both the TIGER 2000 and ARCmap street bases were incorporated by overlaying the sources with the coverage and importing missing features.
c50% of the assembled bases were incorporated into unigenes of 730 bp or longer.
b50% of the assembled bases were incorporated into unigenes of 361 bp or longer.
N50: 50% of the assembled bases were incorporated into sequences with length of N50 or longer.
a50% of the assembled bases were incorporated into contigs of 562 bp or longer.
The mean contig size was 338 bp and the N50 was 474 bp (i.e. 50% of the assembled bases were incorporated into contigs 474 bp or longer).
Similar(42)
That is, various numbers of mismatched bases are incorporated into aptamer-DNA duplex to lower its bonding strength and so as to facilitate aptamer-target interaction during the displacement recognition process.
All the amplified samples from Linköping were pyrosequenced without any verification, as they had been selected from samples known to yield DNA. Pyrosequencing is a real-time sequencing method in which the DNA sequence is identified from light emitted via an enzymatic reaction when bases are incorporated into the DNA molecule [21], [22].
Similarly if the reagent of a previous cycle was not perfectly removed, it is possible that multiple different bases are incorporated, resulting in pre-phasing [ 24].
The isotig N50 length was 1,735 bp (in other words, 50% of the bases are incorporated into isotigs ≥ 1,735 bp), and 14,460 (68.5%) of the isotigs contained only one contig.
We previously noted that edits at a distance from the cleavage site (>10 bases) are incorporated less efficiently than edits closer to the cleavage site (Paix et al. 2014).
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