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Base-by-base conservation scores for human bases were downloaded from UCSC Conservation Track [47] which used phastCons [48] to calculate conservation scores across 28 mammalian species.
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All platforms were 2-channel based, data were downloaded as normalized values, and probes were indexed by gene symbol.
There are 49 genes in total are found to be pathogenic genes of F. graminearum according to PHI-base and were downloaded for future studies.
The normalized occupancies per base pair were downloaded from http://genie.weizmann.ac.il/pubs/nucleosomes08/nucleosomes08_data.html and the predicted occupancies from http://genie.weizmann.ac.il/software/nucleo_genomes.html.html
Sample based genotypes were downloaded for all variants in genomic regions including from 5,000 bp 5-prime upstream to 5000 bp 3-prime downstream of IL-6 and IL-6R independently.
L. loa transcriptome sequencing reads (>5 M spots, 872 M bases) [ 2] were downloaded from NCBI SRA (http://www.ncbi.nlm.nih.gov/sra/SRX130438), trimmed with Trimmomatic [ 27], and assembled with Trinity [ 28] using both ab initio and genome-guided approaches.
Log base 2 data were downloaded, transformed to linear, uploaded to GeneSpring GX 7.3 and then chips and genes were median-normalized.
Gene annotations based on hg18 were downloaded from UCSC Genome Browser database.
The expression profiles of five mammals (Canis lupus familiaris, Homo sapiens, Mus musculus, Rattus norvegicus and Sus scrofa) based on ESTs were downloaded from Unigene ftp http://www.ncbi.nlm.nih.gov/unigene.nih.gov/unigene
Rat microRNAs along with their genomic coordinates based on assembly rn4 were downloaded from miRBase (http://www.mirbase.org/, release 14) [614.
All four groups of genes were collected from previous publications and their sequences were downloaded based on the accession numbers from NCBI http://www.ncbi.nlm.nih.gov/or collected from dissertation text (Dr. Hassan's dissertation, Texas A&M University library).
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