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Polymorphic bases were checked using the original chromatograms in MEGA v7 (Kumar et al. 2016).
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Both vegetative check dams and wire net check dams along with vegetation were used during May June 2009.
Ambiguous bases in coding regions were checked using data in NCBI (http://blast.ncbi.nlm.nih.gov/, last accessed May 13 , 2014 and confirmed by Sanger sequencing.
Purity of compounds were checked using HPLC.
Sequence chromatograms were checked using SeqScape v2.5.
Model assumptions were checked using residual analyses.
Results were checked using the program Tracer1.4.
Primers specific for those sequences were designed manually and the quality of the oligos was checked using web based software Primer 3. (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi).
The stability of the control system is checked using bode diagram based on frequency domain analysis.
Reaction efficiency was checked using standard curves based on a four-fold dilution series of cDNA synthesized from 500 ng of total RNA (1 to 1/256 dilution).
The procedure is checked using Monte Carlo simulations.
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