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1.2 million 454 reads with an average length of 350 bases were assembled into contigs.
After filtering for vector and E. coli sequences, 101,705 reads with a total of 30,549,147 bases were assembled into 803 contigs, 149 of which were > 500 bp and therefore defined as large contigs.
Based on data corresponding to a single run on the GS FLX sequencer, almost 42 million bases were assembled into ~43,000 pieces of putative transcripts and the majority of these have been annotated and functionally classified.
From these data, 847,452 reads (76.6%) with identity tag and length higher than 120 bases were assembled into 42,860 contigs (N50 = 331 bp, consistent with the fraction of the AFLP fragments sequenced: between 200 and 400 bp), leaving 95,812 singletons.
Based on data corresponding to one single run on the FLX Gene Sequencer from 454 Life Science, almost 100 million bases were assembled into ~53,000 pieces of putative transcripts and the majority of these have been annotated and functionally classified.
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A quarter of a sequencing run with the Genome Sequencer FLX Instrument yielded 286,938 reads and 94,447,635 bases that were assembled into ten large contigs (≥ 500 bases) and one small contig (313 bases), indicating a very low number of repetitive sequences in the C. pseudotuberculosis FRC41 genome.
A single run with the Genome Sequencer FLX System yielded 594,627 reads and a total number of 136,924,144 bases that were assembled into 73 large (≥ 500 bases) contigs.
A quarter of a single run with the Genome Sequencer FLX System and Titanium chemistry yielded 273,646 reads with a total number of 112,335,846 bases that were assembled into 73 large (≥ 500 bases) contigs and 19 small contigs.
A quarter of a sequencing run with the Genome Sequencer FLX Instrument and Titanium chemistry yielded 253, 845 reads and 97, 765, 747 bases that were assembled into 93 large contigs (≥500 bases) and 41 small contigs, indicating numerous repetitive elements and insertion sequences in the C. variabile DSM 44702 genome.
After quality filtering, the 454 run generated 864,056 reads, totaling 245,064,986 bases, which were assembled into 14,244 contigs with a threshold of 200 bp overlap and 95% identity.
Sequencing resulted in 2502269 reads with an average length of 239 bases, which were assembled into 29,856 contigs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com