ATP measurement was based on two consecutive reactions; in the first one, ATP is transformed to adenosyl diphosphate (ADP) and 1,3-diphosphoglycerate in the presence of 3-phosphoglycerate phosphokinase); in the following reaction, catalyzed by glyceraldehyde-phosphate-dehydrogenase, 1,3-diphosphoglycerate in the presence of NADH+H is transformed in glyceraldehyde-3-P and NAD+P.
For the second approach, we downloaded experimental nucleosomal probability data from UCSC and defined one nucleosome position of interest within the consensus LINE sequence based on two consecutive crests and troughs for the multiple curves which were spaced approximately 146 bp apart, i.e., one bound nucleosomal DNA length.
The second rotamer library is a two-letter library, in which each rotamer is based on two consecutive amino acid residues (i.e. a dipeptide).
The uplink and downlink channels are assumed to be reciprocal and invariant, and are based on two consecutive equal time slots.
A two-step surface modification strategy based on two consecutive photoinduced thiol ene reactions has been developed which enables controlled and patterned immobilization of micro-scaled inorganic particles onto diene-rubber surfaces.
Disease activity across the 12 months was categorized as consistently inactive, consistently active, or fluctuating between inactive and active, based on two consecutive six-month MIBDI assessments.
The volumetric analysis, using the FreeSurfer software package (version 4.5.0) [ 38– 40] was based on two consecutive T1-volumes acquired during a single examination.
We believe that this definition is more informative than the traditional based on two consecutive HPV-positive tests that can be separated by irregular time intervals.
Based on two consecutive measurements, an index of cumulative job stress was constructed and its associations with five indicators of mental health were studied, excluding caseness at entry (for depression, anxiety, somatisation, chronic fatigue and psychotropic drug consumption respectively).
Other strengths include the well-defined phenotype of the population, with albuminuria status based on two consecutive 24-h urine collections, the rigorous definitions for transition in albuminuria stage, and the availability of samples that had never been thawed.
