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This can be attributed to higher percentage of usable bases on target, as calculated with HsMetrics.
The percent usable bases on target are lower in the whole-exome WGA subset than the whole exome genomic sequencing experiment.
For both sequencing experiments a large percentage of reads were marked as duplicates, as the percentage of usable bases on target for each of the capture experiments does not exceed 40%.
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Six pairs of gene-specific primers (five candidate genes and one actin gene) were designed based on target gene sequences using Primer Express software (Additional file 1: Table S1).
Also based on target prediction and degradome data-based validation, certain target mimics were identified to be regulated by specific miRNAs.
We tested two different genome shuffling strategies, one based on random mating and one based on targeted mating.
MCS selection based on target relaxation and target increment.
An alternative approach, based on target doping, is presented in this paper.
Figure 2 Output response of system ( 1 ) under delay-independent control based on target tracking.
Figure 6 Output response of system ( 1 ) under delay-dependent control based on target tracking.
The performance analysis is based on target detection reliability and living nodes as time increases.
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