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To explore the effects of low coverage of individual bases on detection of methylation difference, we filtered cytosines that had observed read depths of less than a specific low-count threshold (i.e., 5, 7, or 10) for either sample (Col-0 and met1 3).
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Other authors have described systems based on detection of one or both annihilation photons, at cost of a higher sensitivity to background radioactivity.
The assay detects the cyanogenesis reaction and is based on detection of released HCN via a Feigl Anger test paper30.
Herein a DNA-based assay is described that enables tissue identification based on detection of tissue-specific methylation patterns.
Such deletion products were detected by mass spectrometry and were expected based on detection of truncated products from P-labeled template-primers by polyacrylamide gel electrophoresis.
Hence, there are basically three classes of detectable markers directly derived from HPVs: molecular markers based on detection of nucleic acid sequences, serological markers based on detection of antibodies against viral proteins, and cellular markers based on detection of proteins expressed intracellularly, upon either infection or carcinogenesis.
In endemic areas, diagnosis may be based on detection of antibody but in new introductions of disease it is preferable to detect the virus.
The remaining nuclei were separated based on detection of AlexaFluor 488-conjugated anti-NeuN antibody.
Microarray analysis is commonly based on detection of fluorescence signals through a confocal laser scanner.
Early diagnosis of HCV infection is based on detection of antibodies against HCV proteins using recombinant viral antigens.
The most reliable and accurate methods are based on detection of artificial markers placed in the environment.
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