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Venter expects to churn out a whopping 100 million bases of sequence data per day, every day.
Whole genome shotgun sequencing was performed on the Illumina platform generating 142 billion bases of sequence data.
A total of 524 million reads corresponding to 52.9 billion bases of sequence data were generated from the 17 RNA samples.
Protocol and platform engineering improvements have enabled the generation of 1 × 10 9 bases of sequence data in 27 h for ∼$1000 (Quail et al., 2012).
The MiSeq flowcell of 2 × 250 bp paired-end reads resulted in 15 to 17 million read pairs corresponding to 5.8 to 8.5 billion bases of sequence data.
We obtained genomic DNA from the reference rhesus macaque (animal 17573) [ 1] and performed whole genome Illumina sequencing on a GAIIx instrument, yielding 107 billion bases of sequence data.
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Parameters for Bowtie were set to exclude the first 10 bases and last 30 bases of sequencing data leaving 60 bases of high quality sequence for alignment.
The analysis is based on up to 12896 base pairs of sequence data per species (average = 2497 bp) from 12 genes, including seven nuclear loci (BDNF, c-mos, NT3, PDC, R35, RAG-1, and RAG-2), and five mitochondrial genes (12S, 16S, cytochrome b, ND2, and ND4).
A total of 42599 base pairs of sequence data covering Gag, Pol and Nef were exchanged and analyzed using site-specific software and procedures in a blinded fashion.
This is theoretically possible using 454 pyrosequencing, which generates billions of base pairs of sequence data.
This generated a total of 7.4 × 106 base-pairs of sequence data in 203,108 sequencing reads.
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