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Once the average quality score in the window was below a threshold score of 15, the EST was split and the longest remaining sequence retained as the trimmed EST. EST sequences with less than 100 bases of high quality sequence were removed.
Parameters for Bowtie were set to exclude the first 10 bases and last 30 bases of sequencing data leaving 60 bases of high quality sequence for alignment.
Once the average quality score in the window was below the threshold (Q15) the EST was split and the longest remaining sequence segment was retained as the trimmed EST. EST sequences with less than 100 bases of high quality sequence were removed.
Estimated high quality coverage calculated by dividing the number of reads with at least the first 30 bases of high quality by the estimated genome size (of cultured fungi) or genome sizes (of lichenizing fungus and lichenizing alga or cyanobacterium but excluding the associated microbial community, and therefore overestimating coverage).
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Eben Upton said the UK tech industry has a powerful base of high quality engineers and academic research, investment funding and business networks, but that the government could do more regionally.
To build a knowledge base of high quality, effective methods have to be in place to catch inconsistencies in graph-structured data.
To assess the quality of the studies, we applied a quality rating scale, and determined (using a priori definitions of high, medium, and low quality) that the evidence base was of high quality.
Not very many years ago a graduate student using a slab gel electrophoresis instrument with fluorescent terminator chemistry would be excited to get 500-800 base pairs of high quality sequence data from a single gene after about a week's worth of work.
In this study, we generated over three billion bases of high-quality DNA sequence using Illumina technology and demonstrated the suitability of short-read sequencing for the de novo assembly and annotation of genes expressed in a eukaryote without prior genome information.
In this study, we generated over one billion bases of high-quality DNA sequence for MEAM1 with Illumina technology.
In this study, we generated more than four billion bases of high-quality DNA sequence and obtained 70,392 unigenes from the Sacha Inchi seed transcriptome.
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