Sentence examples for bases of cDNA from inspiring English sources

e) The last 50 bases of cDNA.

The regions analyzed were: a) The first 50 bases of cDNA.

We used 454 pyrosequencing to sequence over 500 million bases of cDNA from the ovaries and embryos of the milkweed bug Oncopeltus fasciatus, which lacks a sequenced genome.

In this study we used an ultra-high throughput pyrosequencing approach [ 18] to quickly generate millions of bases of cDNA sequence for plant transcriptome analysis [ 19- 22].

Studies have already demonstrated that sequencing tags (typically 20 36 bases of cDNA) produces more robust results and detects more differentially expressed genes than several different microarray platforms, particularly when using a con-specific reference genome/transcriptome to which tags can be aligned [ 5].

Although the use of next generation sequencer helped in making advances in analyzing TSSs, it still remains extremely difficult to evaluate diversities of mRNAs transcribed by each gene because of their accumulation of short-length sequences (less than 50 bases) of cDNA clones.

In nrITS we did not observe significant differences between the frequency of allele-specific bases of cDNA-pools and genomic DNA in any of the alleles, so that the null hypothesis of equal transcription was not rejected.

Here, the use of microarrays based on cDNA libraries of biological samples enriched in endosperm tissue allowed us to identify with a good level of confidence a large collection of genes differentially expressed in endosperm mutants that were not previously identified through traditional analyses and in a similar study as reported previously [ 7].

The use of an oligo-capping technique allows TSS to be assigned to independent genomic positions to which the first bases of sequenced cDNA were mapped, thus most cDNA sequence 5' ends should correspond to active TSSs [43].

However, the number of WC1 genes in the multi-gene family agrees reasonably well with previous reports estimating the occurrence of many related WC1 genes based on Southern blots [ 11] and of 13 WC1 genes based on cDNA analysis of intracytoplasmic tail region transcripts [ 48] but is fewer than the 50 WC1 genes predicted for sheep by Southern blotting [ 46, 47].

For example, if 62 out of the total of 100 bases of a cDNA aligned perfectly and the other 38 bases did not align, the overall identity is 62%.