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Consensus sequence of splice site is bases of alignment of known human donor and acceptor site sequences extracted from EID (Exon-Intron database).
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The most 5' primer was also based on alignment of multiple copies of the A repeat from Mus musculus and three Microtus species [7], [10].
If required, a redefinition of primers based on alignment of the DNA sequences obtained and those used for the PHYLORPH search could help to extend this sequencing to more distantly related species.
Selective constraint is calculated based on alignment of orthologous IGRs and introns of T. parva and T. annulata.
A pair of binding sites is then compared based on alignment of 90 lists of sorted distances obtained for each of the sites.
Classification of HKs is usually based on alignment of residues surrounding the H-box that contains the conserved His residue within the histidine kinase domain (HisKA) [ 9, 40].
(A ) Phylogeny based on alignment of 113aa without indels consisting of top E-value hits to blastp using WORiA phage lysozyme as a query.
Phylogeny based on alignment of 186aa without indels consisting of top E-value hits to blastp using A. oryzae lysozyme as a query.
(A ) App-1 phylogeny based on alignment of 141aa without indels consisting of top E-value hits to blastp using A. boonei App-1 as the query.
Based on alignment of conserved sequences upstream of operons involved in thiamine biosynthesis from various bacteria, a putative THI-box sequence (5'-ACCCTTTGAACCTGATCTAGTTAGCACTAGTGTAGG-3') was identified upstream of thiC in strains TX07-7308 ATCCATCC 25017.
An hypothetical F4 site was also located upstream of ompN in Photorhabdus based on alignment of sequences with the nt positions and sequence described for the E. coli F4 [ 29].
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