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amotif ± 1 bp b motif in non-coding strand corresponding to these bases in the coding strand.
The 5' primer and the 3' primer for HLA-DQB1 exon 2 extend 9, respectively 2 bases in the coding region.
The GC content of each gene was estimated as the proportion of GC bases in the coding regions annotated in the reference D. melanogaster genome.
In most cases the strand alignment of the open reading frames is supported by the apparent change in strand AG content, with a distinct preference for A and G bases in the coding strand.
This value is equal to the ratio of the base number in a gene covered by unique mapping reads to the total number of bases in the coding region of that gene [ 32, 33].
To do so, the degree of overlap between SYT-SSX2 and a given modification was calculated as a ratio of bases covered per 5kb bin upstream of the TSS or the ratio of bases covered in the gene body over the total number of bases in the coding sequence.
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The c.1A>G affects the first base in the coding sequence and might be expected to ablate the initiation of ATM protein translation altogether.
The left arm XhoI site is located 93 bases 5′of the NRARP protein ATG start codon, while the BamHI site in the right arm is 1,286 bases 3′of the last base in the coding sequence of the NRARP protein.
The Nrarp tm1Grid targeting vector was constructed from strain 129S6/Sv BAC clones, and deletes the entire coding sequence of the NRARP protein (from 93 base pairs 5′of the ATG start site to 1,286 bases 3′of the last base in the coding sequence of the NRARP protein).
However, information on DNA based variations in the coding and non-coding regions and information on SNPs (Single Nucleotide Polymorphisms) and insertions/deletions (INDELs) of one to several hundred base pairs, thus far have not been studied.
SNP density was calculated as the number of SNPs per 1,000 bases (kb) in the coding regions of toxin and nontoxin transcripts containing SNPs [ 35].
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