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Between-group differences at baseline were determined with the Kruskal-Wallis test.
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The level of statistical significance for weight loss at each time-point during the follow-up, compared to baseline, was determined with a paired-samples t-test.
The correlations between skin AF and HbA1c AUC for the past 3, 5, 10, 15, and 20 years from baseline were determined in patients with type 1 diabetes (Fig. 2).
Neuronal tuning width, tuning amplitude and tuning baseline were determined by fitting the neuronal responses with a wrapped Gaussian.
Floor or ceiling effects at baseline were determined to assess the proportions of patients with the minimum possible (floor) or maximum possible (ceiling) values for individual FSS items and FSS total scores, and were considered present when more than 14.3% (calculated as 100/7, reflecting the 7 possible response categories for the FSS) of patients achieved these values.
Baseline values were determined with samples collected from animals in the control group at each time point.
Plasma insulin at baseline was determined in duplicate with the Phadebas Radioimmunoassay kit (Pharmacia Diagnostics Inc., Piscataway, NJ).
Haplotype effects on baseline log-transformed CRP levels were determined with linear regression.
Baseline and threshold cycles (Ct) were determined with 2nd maximum derivative method using the LightCycler ® 480 Software release 1.5.0.
Statistical differences between Day 1 (baseline) and Day 2 (24 h) were determined with a paired t test and, if not normally distributed, with the Wilcoxon signed rank test.
Differences in baseline characteristics and PROM scores were determined with a student's t-test for continuous variables, and with a Chi-square or Fisher's exact test where appropriate for categorical variables.
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