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Statistically significant follow up differences or effects from baseline were determined using Bonferroni multiple comparison test.
Statistical differences between the groups at baseline were determined using a Kruskal-Wallis test on ranks.
Differences between offspring and control groups at baseline were determined using unpaired t tests.
Associations between flavan-3-ol intake and blood pressure at baseline were determined using linear regression models.
Changes in these values from baseline were determined using a mixed effect regression model that took into account repeated measures within-patient and were adjusted for the period and period-treatment interaction effects.
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Since UA recall is more appropriate for unbalanced datasets, statistical significance over the baseline is determined using a one-tailed test (difference of proportions) over the UA recall values.
The blood glucose baseline was determined using Roche ACCU-CHEK Aviva (Germany, #04528280001) blood glucose monitor and strips.
Data from all baseline testing sessions were pooled and the statistical significance of PPI at baseline was determined using a Wilcoxon rank-sum test to a 95% confidence rating for each GP at each background frequency, to ensure stable PPI was present prior to noise exposure.
HIV sero-status at baseline was determined using NACO HIV testing guidelines via rapid tests: HIV-negative status was based on a single highly sensitive rapid test (DetermineTM HIV-1/2; Inverness Medical Japan Co Ltd., Japan) and a positive result on two additional confirmatory rapid tests (SignalTM HIV-1/2; Span Diagnostics, Surat India and TridotTM; Biomed Industries, Himachal Pradesh, India).
The threshold cycle data (CT) and baselines were determined using auto settings.
In all the qPCR assays, the threshold cycle data (Ct) and baselines were determined using auto settings.
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