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To obtain standard curves with maximal dynamic range, baseline specimens were used as standards for vDNA-measurements.
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Preinoculation baseline serum specimens were used as negative controls for each monkey.
To conserve baseline samples, stored specimens from the year 3 follow-up visit were used, except for participants with inadequate specimen volume (n = 70), for whom baseline stored specimens were used.
Snap-frozen specimens were also available in a subset of cases, and these specimens were used for hormone assays (baseline samples: n=2, post-treatment samples: n=8) and/or microarrary analysis (baseline samples: n=2, post-treatment samples: n=5).
Patient blood specimens were used without spiking.
Small-size specimens were used.
Normal skin specimens were used as controls.
Otherwise, fresh stool specimens were used.
These specimens were used for LOH analysis.
A plate-type specimen was used for, and a disk-type specimen was used for.
Baseline specimens were selected during January May and all postpandemic specimens from September 2009.
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